Using Automated
Electrophoresis to Assess RNA Quality
HERCULES,
Calif., March 14, 2008 – Bio-Rad
Laboratories, Inc. (AMEX: BIO and BIOb), a multinational manufacturer and
distributor of life science research and clinical diagnostic products,
announced today the availability of a technical note (#5315A), which describes
the use of Bio-Rad’s Experion™ automated electrophoresis system to
assess RNA quality and quantity in siRNA-induced gene silencing experiments.
An awareness of the quality of an RNA sample is essential to the success of experiments designed to study gene expression. Failure to evaluate the state of RNA samples before their use in downstream applications, such as validation of siRNA gene silencing using RT-qPCR, can yield faulty conclusions.
The Experion system has been shown to be a useful tool in this regard, accurately quantitating RNA as well as providing information on the overall quality and integrity of the RNA that will ultimately be analyzed in various downstream applications.
In this paper, it is
discussed how the Experion system, which uses microfluidics technology, can be
incorporated into an RNAi experimental workflow. Further, it is shown that the
qualitative and quantitative RNA sample information that is obtained using this
system is beneficial to the successful evaluation and validation of the
efficacy of siRNA-mediated gene silencing.
Supported be numerous graphs, figures and tables, the four-page technical note provides the methods, results, and discussion for assessing RNA quality and quantity, as well as illustrating that RNA quality is critical to downstream results.
Availability
The
technical note (number 5315A) is available either from a local Bio-Rad sales
office or may be downloaded at www.bio-rad.com.
About Bio-Rad
Bio-Rad Laboratories, Inc. (AMEX: BIO and BIOb) has remained at the center of
scientific discovery for more than 50 years manufacturing and distributing a
broad range of products for the life science research and clinical diagnostic
markets. The company is renowned worldwide among hospitals, universities, major
research institutions, as well as biotechnology and pharmaceutical companies
for its commitment to quality and customer service. Founded in 1952, Bio-Rad is
headquartered in Hercules, California, and serves more than 85,000 research and
industry customers worldwide through its global network of operations. The
company employs approximately 6,300 people
globally and had revenues of nearly $1.3 billion in 2006. For more information,
visit www.bio-rad.com.
For more
information, please contact:
Mary Grace Brubacher
Bio-Rad Laboratories, Inc.
510-741-5347