Bio-Rad Offers Real-Time PCR Technology for Forensics
DNA Analysis
HERCULES, Calif., June 12, 2008 Bio-Rad Laboratories, Inc. (AMEX: BIO and BIOb), a multinational
manufacturer and distributor of life science research products and clinical
diagnostics, today announced the availability of Bio-Rads bulletin (number
5563) entitled Biotechnology Explorer
Crime Scene Investigator PCR Basics
Kit: A Real-Time PCR
Extension. Developed for conventional PCR and
end-point analysis of amplification products by gel electrophoresis, the kit
can also be easily adapted for instruction in real-time PCR.
Bulletin
5563 was designed as a teaching tool to help instructors teach students the principles of the
polymerase chain reaction (PCR) and its use in forensic DNA analysis. By the
end of the exercise described in the bulletin, students will:
·
Better understand key differences between the goals of
conventional PCR and real-time PCR analysis
·
Be able to analyze and evaluate real-time PCR results
·
Understand the advantage of melt-curve analysis of the SYBR Green
I detection chemistry and be able to compare this with final amplification
products using agarose gel analysis
·
Be able to determine the accuracy and reliability of pipeting
techniques by preparing duplicate or triplicate serial dilutions of template
DNA
·
Discover the sensitivity of PCR and how little template is required
for detectable amplification results
·
Understand how real-time PCR can quantitate the DNA in a sample
·
Have developed an understanding of the molecular basis of DNA
amplification reactions using real-time thermal cyclers
Application Note
Availability
Bulletin
5563 is available either from a local Bio-Rad sales office or can be downloaded
at http://www.biorad.com/cmc_upload/Literature/208684/Bulletin_5563.pdf
About
Bio-Rad
Bio-Rad
Laboratories, Inc. (AMEX: BIO and BIOb) has remained at the center of
scientific discovery for more than 50 years manufacturing and distributing a
broad range of products for the life science research and clinical diagnostic
markets. The company is renowned worldwide among hospitals, universities, major
research institutions, as well as biotechnology and pharmaceutical companies
for its commitment to quality and customer service. Founded in 1952, Bio-Rad is
headquartered in Hercules, California, and serves more than 85,000 research and
industry customers worldwide through its global network of operations. The
company employs approximately 6,300 people globally and had revenues
approaching $1.5 billion in 2007. For more information, visit www.bio-rad.com.
For more
information contact:
Laurie Usinger
Bio-Rad Laboratories, Inc.
510-724-6665